{"id":401730,"date":"2024-10-20T04:57:47","date_gmt":"2024-10-20T04:57:47","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-175042022\/"},"modified":"2024-10-26T08:47:17","modified_gmt":"2024-10-26T08:47:17","slug":"bs-en-175042022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-175042022\/","title":{"rendered":"BS EN 17504:2022"},"content":{"rendered":"
This document specifies a method for the determination of free gossypol, extractable by acidified acetonitrile\/water, in cottonseed, cottonseed products and compound feeds by liquid chromatography with tandem mass spectrometry (LC-MS\/MS). The method described in this document has been successfully validated in the range of 69 mg\/kg to 5 950 mg\/kg by collaborative trial in the following matrices: cottonseed, cottonseed products (cake\/meal, hulls) and compound feeds for bovine, porcine and poultry. NOTE It is possible to reach quantification limits of approximatively 5 mg\/kg in compound feeds. The method might be applicable at lower and at higher concentrations than the concentration range validated in the collaborative trial. However, this needs to be assessed by in-house validation.<\/p>\n
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2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 6 Apparatus <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 7 Procedure 7.1 General 7.2 Sample pre-treatment <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 7.3 Test portion 7.4 Extraction <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 8 LC-MS\/MS analysis 8.1 General <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 8.2 Analysis sequence 9 Results 9.1 Identification <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 9.2 Quantification <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | 10 Precision 10.1 General 10.2 Repeatability 10.3 Reproducibility <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | 11 Test report <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | Annex A (informative)Precision data <\/td>\n<\/tr>\n | ||||||
20<\/td>\n | Annex B (informative)Example of LC-MS\/MS conditions B.1 General B.2 HPLC conditions <\/td>\n<\/tr>\n | ||||||
21<\/td>\n | B.3 MS conditions <\/td>\n<\/tr>\n | ||||||
22<\/td>\n | Annex C (informative)Example chromatogram gossypol in compound feed <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Animal feeding stuffs: Methods of sampling and analysis. Determination of gossypol in cotton seed and feeding stuff by LC-MS\/MS<\/b><\/p>\n |