{"id":379611,"date":"2024-10-20T03:03:14","date_gmt":"2024-10-20T03:03:14","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-237322021\/"},"modified":"2024-10-26T05:29:21","modified_gmt":"2024-10-26T05:29:21","slug":"bs-iso-237322021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-237322021\/","title":{"rendered":"BS ISO 23732:2021"},"content":{"rendered":"

This document specifies a general protocol for the observation of the meiofaunal community in the deep seabed.<\/p>\n

The standardized method can be used in any phase [baseline data acquisition, monitoring during and after mining (testing)] accompanying resource development, making it easier to compare data beyond differences in workers and waters.<\/p>\n

This document is intended for marine environment impact assessments and other occasions where long-term image-based data are required.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Principle
5 Sampling
5.1 Method and equipment <\/td>\n<\/tr>\n
11<\/td>\n5.2 Sub-sampling
5.3 Sample preservation
5.3.1 Preservation method before analysis
5.3.2 Preservation method after analysis
5.4 Recording sample information
6 Procedures for imaging flow cytometry (morphological analysis)
6.1 Pre-processing of samples
6.2 Observation using imaging flow cytometer
6.3 Image processing <\/td>\n<\/tr>\n
12<\/td>\n7 Procedures for metagenomic analysis (molecular analysis)
7.1 General
7.2 Nucleic acids (DNA\/RNA) extraction
7.3 PCR primers
7.4 PCR protocol
7.4.1 DNA
7.4.2 RNA (cDNA library production)
7.5 Check and purification of PCR amplicon <\/td>\n<\/tr>\n
13<\/td>\n7.6 Index PCR
7.7 Purification of amplicon by magnetic beads
7.8 Concentration measurement
7.9 Quality check for amplicon by an automated electrophoresis system using microfluidic chip
7.10 Sequencing by a next generation sequencer
7.11 Data processing
8 Data and sample management
9 Combination of morphological analysis and molecular analysis (integrated analysis) <\/td>\n<\/tr>\n
15<\/td>\nAnnex A (informative) Example procedure for the analysis of the meiofaunal community by imaging flow cytometry <\/td>\n<\/tr>\n
18<\/td>\nAnnex B (informative) Example procedure for analysis of meiofaunal community by NGS <\/td>\n<\/tr>\n
21<\/td>\nAnnex C (informative) Example procedure for quantitative analysis of meiofaunal community by NGS <\/td>\n<\/tr>\n
24<\/td>\nAnnex D (informative) Useful information for users <\/td>\n<\/tr>\n
25<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Marine technology. Marine environment impact assessment (MEIA). General protocol for observation of meiofaunal community<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2021<\/td>\n28<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":379621,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[186,2641],"product_tag":[],"class_list":{"0":"post-379611","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-13-020-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/379611","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/379621"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=379611"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=379611"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=379611"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}