{"id":340866,"date":"2024-10-19T23:53:18","date_gmt":"2024-10-19T23:53:18","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-iso-ts-15216-12013\/"},"modified":"2024-10-25T23:04:12","modified_gmt":"2024-10-25T23:04:12","slug":"bsi-pd-cen-iso-ts-15216-12013","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-iso-ts-15216-12013\/","title":{"rendered":"BSI PD CEN ISO\/TS 15216-1:2013"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
9<\/td>\nIntroduction <\/td>\n<\/tr>\n
11<\/td>\nSection sec_1
Section sec_2
Section sec_3
Section sec_3.1
Section sec_3.2
Section sec_3.3
1\tScope
2\tNormative references
3\tTerms and definitions <\/td>\n<\/tr>\n
12<\/td>\nSection sec_3.4
Section sec_3.5
Section sec_3.6
Section sec_3.7
Section sec_3.8
Section sec_3.9
Section sec_3.10
Section sec_3.11
Section sec_3.12 <\/td>\n<\/tr>\n
13<\/td>\nSection sec_3.13
Section sec_3.14
Section sec_3.15
Section sec_3.16
Section sec_3.17
Section sec_3.18
Section sec_4
Section sec_4.1
Section sec_4.2
4\tPrinciple
4.1\tVirus extraction
4.2\tRNA extraction <\/td>\n<\/tr>\n
14<\/td>\nSection sec_4.3
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.4.3
4.3\tReal-time reverse transcription polymerase chain reaction (real time RT-PCR)
4.4\tControl materials <\/td>\n<\/tr>\n
15<\/td>\nSection sec_4.5
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.2.1
Section sec_5.2.2
Section sec_5.2.3
Section sec_5.2.4
Section sec_5.2.5
Section sec_5.2.6
Section sec_5.2.7
Section sec_5.2.8
Section sec_5.2.9
Section sec_5.2.10
Section sec_5.2.11
Section sec_5.2.12
Section sec_5.2.13
Section sec_5.2.14
Section sec_5.2.15
Section sec_5.2.16
4.5\tTest results
5\tReagents
5.1\tGeneral
5.2\tReagents used as supplied <\/td>\n<\/tr>\n
16<\/td>\nSection sec_5.2.17
Section sec_5.2.18
Section sec_5.2.19
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.3.4
Section sec_5.3.5
Section sec_5.3.6
Section sec_5.3.7
Section sec_5.3.8
5.3\tPrepared reagents <\/td>\n<\/tr>\n
17<\/td>\nSection sec_5.3.9
Section sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.3
Section sec_6.4
Section sec_6.5
Section sec_6.6
Section sec_6.7
Section sec_6.8
Section sec_6.9
Section sec_6.10
Section sec_6.11
Section sec_6.12
Section sec_6.13
6\tApparatus and materials <\/td>\n<\/tr>\n
18<\/td>\nSection sec_6.14
Section sec_6.15
Section sec_6.16
Section sec_6.17
Section sec_6.18
Section sec_6.19
Section sec_6.20
Section sec_6.21
Section sec_6.22
Section sec_6.23
Section sec_6.24
Section sec_6.25
Section sec_6.26
Section sec_6.27
Section sec_6.28
Section sec_7
Section sec_8
Section sec_8.1
7\tSampling
8\tProcedure
8.1\tGeneral laboratory requirements <\/td>\n<\/tr>\n
19<\/td>\nSection sec_8.2
Section sec_8.2.1
Section sec_8.2.2
Section sec_8.2.3
Section sec_8.2.4
8.2\tVirus extraction <\/td>\n<\/tr>\n
20<\/td>\nSection sec_8.2.5
Section sec_8.2.6 <\/td>\n<\/tr>\n
21<\/td>\nSection sec_8.3
Section sec_8.4
Section sec_8.4.1
Section sec_8.4.2
Section sec_8.4.2.1
8.3\tRNA extraction
8.4\tReal-time RT-PCR <\/td>\n<\/tr>\n
22<\/td>\nSection sec_8.4.2.2
Section sec_8.4.2.3 <\/td>\n<\/tr>\n
23<\/td>\nSection sec_8.4.2.4
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.3
9\tInterpretation of results
9.1\tGeneral
9.2\tConstruction of standard curves
9.3\tCalculation of amplification efficiency <\/td>\n<\/tr>\n
24<\/td>\nSection sec_9.4
Section sec_9.5
9.4\tCalculation of extraction efficiency
9.5\tSample quantification <\/td>\n<\/tr>\n
25<\/td>\nSection sec_9.6
Section sec_10
Section sec_11
9.6\tTheoretical limit of detection
10\tExpression of results
11\tTest report <\/td>\n<\/tr>\n
27<\/td>\nAnnex sec_A
Annex\u00a0A
\n(normative)<\/p>\n

Diagram of procedure <\/td>\n<\/tr>\n

28<\/td>\nAnnex sec_B
Table tab_B.1
Table tab_B.2
Annex\u00a0B
\n(informative)<\/p>\n

Real-time RT-PCR mastermixes and cycling parameters <\/td>\n<\/tr>\n

29<\/td>\nAnnex sec_C
Annex sec_C.1
Annex sec_C.2
Annex\u00a0C
\n(informative)<\/p>\n

Real-time RT-PCR primers and hydrolysis probes for the detection of HAV, norovirus GI and GII and mengo virus (process control) <\/td>\n<\/tr>\n

30<\/td>\nAnnex sec_C.3
Annex sec_C.4 <\/td>\n<\/tr>\n
31<\/td>\nAnnex sec_D
Annex sec_D.1
Annex sec_D.2
Annex sec_D.2.1
Annex sec_D.2.2
Annex sec_D.3
Annex\u00a0D
\n(informative)<\/p>\n

Growth of mengo virus strain MC0 for use as a process control <\/td>\n<\/tr>\n

32<\/td>\nAnnex sec_E
Annex sec_E.1
Annex sec_E.1.1
Annex sec_E.1.2
Annex sec_E.2
Annex sec_E.2.1
Annex sec_E.2.2
Annex sec_E.2.3
Annex sec_E.2.4
Annex sec_E.3
Annex\u00a0E
\n(informative)<\/p>\n

RNA extraction using the BioMerieux NucliSens\u00ae system
Annex\u00a0E
\n(informative)<\/p>\n

RNA extraction using the BioMerieux NucliSens\u00ae system <\/td>\n<\/tr>\n

34<\/td>\nAnnex sec_F
Annex sec_F.1
Annex sec_F.1.1
Annex sec_F.1.2
Annex sec_F.2
Annex sec_F.2.1
Annex sec_F.2.2
Annex sec_F.3
Annex sec_F.3.1
Annex sec_F.3.2
Annex\u00a0F
\n(normative)<\/p>\n

Composition and preparation of reagents and buffers
Annex\u00a0F
\n(normative)<\/p>\n

Composition and preparation of reagents and buffers <\/td>\n<\/tr>\n

35<\/td>\nAnnex sec_F.4
Annex sec_F.4.1
Annex sec_F.4.2
Annex sec_F.5
Annex sec_F.5.1
Annex sec_F.5.2 <\/td>\n<\/tr>\n
36<\/td>\nAnnex sec_G
Annex sec_G.1
Annex sec_G.2
Annex sec_G.2.1
Annex sec_G.2.2
Annex sec_G.2.3
Annex sec_G.2.4
Annex sec_G.2.5
Annex sec_G.2.6
Annex sec_G.2.7
Annex sec_G.2.8
Annex sec_G.3
Annex sec_G.4
Annex\u00a0G
\n(informative)<\/p>\n

Generation of double-stranded DNA (dsDNA) control stocks
Annex\u00a0G
\n(informative)<\/p>\n

Generation of double-stranded DNA (dsDNA) control stocks <\/td>\n<\/tr>\n

37<\/td>\nAnnex sec_G.5 <\/td>\n<\/tr>\n
38<\/td>\nAnnex sec_H
Annex sec_H.1
Annex sec_H.2
Annex sec_H.2.1
Annex sec_H.2.2
Annex sec_H.2.3
Annex sec_H.2.4
Annex sec_H.2.5
Annex sec_H.2.6
Annex sec_H.2.7
Annex sec_H.2.8
Annex sec_H.3
Annex sec_H.4
Annex\u00a0H
\n(informative)<\/p>\n

Generation of external control RNA (EC RNA) stocks
Annex\u00a0H
\n(informative)<\/p>\n

Generation of external control RNA (EC RNA) stocks <\/td>\n<\/tr>\n

39<\/td>\nAnnex sec_H.5
Annex sec_H.6 <\/td>\n<\/tr>\n
40<\/td>\nAnnex sec_I
Annex\u00a0I
\n(informative)<\/p>\n

Typical optical plate layout
Annex\u00a0I
\n(informative)<\/p>\n

Typical optical plate layout <\/td>\n<\/tr>\n

41<\/td>\nReference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Reference ref_6
Reference ref_7
Reference ref_8
Reference ref_9
Reference ref_10
Reference ref_11
Reference ref_12
Bibliography
Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of food and animal feed. Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR – Method for quantification<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2013<\/td>\n44<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":340870,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-340866","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/340866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/340870"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=340866"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=340866"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=340866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}