{"id":338611,"date":"2024-10-19T23:41:50","date_gmt":"2024-10-19T23:41:50","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-233052020\/"},"modified":"2024-10-25T22:48:08","modified_gmt":"2024-10-25T22:48:08","slug":"bs-iso-233052020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-233052020\/","title":{"rendered":"BS ISO 23305:2020"},"content":{"rendered":"

This document specifies a method for the quantitative determination of biotin and\/or biocytin in fortified milk powders, infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using liquid chromatography coupled with immunoaffinity column clean-up extraction.<\/p>\n

Precision data from an interlaboratory study is given in Annex B<\/span>. A comparison between data obtained with the method in this document and EN 15607<\/span><\/span> is given in Annex C<\/span>.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Principle <\/td>\n<\/tr>\n
8<\/td>\n5 Reagents and materials
5.1 General
5.1.1 Laboratory reagent grade water.
5.1.2 Sodium dihydrogen phosphate dihydrate (CAS # 13472-35-0).
5.1.3 Disodium hydrogen phosphate dihydrate (CAS # 10028-24-7).
5.1.4 Sodium hydroxide (CAS #1310-73-2).
5.1.5 Methanol, HPLC grade (CAS # 67-56-1).
5.1.6 Acetonitrile, HPLC grade (CAS # 75-05-8).
5.1.7 Ortho-phosphoric acid, mass fraction w (H3PO4) = 85 % (CAS # 7664-38-2).
5.2 Reagent preparation <\/td>\n<\/tr>\n
9<\/td>\n5.3 Standard preparation <\/td>\n<\/tr>\n
10<\/td>\n5.4 Calculation of concentration
6 Apparatus
6.10 Horizontal shaker. <\/td>\n<\/tr>\n
11<\/td>\n6.18 Vortex mixer.
7 Procedure
7.1 Sample preparation <\/td>\n<\/tr>\n
12<\/td>\n7.2 Chromatography <\/td>\n<\/tr>\n
13<\/td>\n7.3 Quality control
8 Calculations <\/td>\n<\/tr>\n
14<\/td>\n9 Precision
9.1 General
9.2 Repeatability
9.3 Reproducibility
10 Uncertainty of measurement
11 Limit of quantitation <\/td>\n<\/tr>\n
15<\/td>\nAnnex A (informative) Example chromatograms <\/td>\n<\/tr>\n
17<\/td>\nAnnex B (informative) Precision data <\/td>\n<\/tr>\n
19<\/td>\nAnnex C (informative) Comparison between this document and EN 15607 <\/td>\n<\/tr>\n
21<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Fortified milk powders, infant formula and adult nutritionals. Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2020<\/td>\n22<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338615,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[960,2641],"product_tag":[],"class_list":{"0":"post-338611","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-67-100-10","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338611","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338615"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338611"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338611"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338611"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}