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BSI PD ISO/TS 4988:2022

BSI PD ISO/TS 4988:2022

$142.49

Nanotechnologies. Toxicity assessment and bioassimilation of manufactured nano-objects in suspension using the unicellular organism Tetrahymena sp.

Published By Publication Date Number of Pages
BSI 2022 24
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PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
11 4 Abbreviated terms
12 5 Materials
5.1 Test organism and culture medium
5.2 Chemicals
5.2.1 General chemicals
5.2.2 Additional chemicals for nutrient medium
13 6 Technical equipment
7 Preparation and characterization of the nano-object
7.1 Nano-object characterization
14 7.2 Dispersion preparation
7.3 Dispersion characterization
7.4 Preparation of media for toxicity tests
8 Culture of Tetrahymena sp.
8.1 General
8.2 Tetrahymena culturing conditions
8.2.1 Tetrahymena growth conditions
15 8.2.2 Tetrahymena conditions during exposure
9 Effect of nano-objects on Tetrahymena sp.
9.1 Test concentrations
9.1.1 Range finding test
9.1.2 Definitive test
16 9.2 Duration
9.3 Observations
9.4 Detailed description of exposure condition
17 9.5 Toxicity assessments
9.5.1 Cell viability
9.5.2 Population growth impairment tests
9.5.3 ATP assay
9.5.4 MTT assay
18 9.5.5 LDH assay
9.6 Phagocytic activity and material bioassimilation
10 Data analysis
11 Test report
19 12 Results validity with negative control
20 Bibliography

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BSI PD ISO/TS 4988:2022
$142.49