BS ISO 23305:2020

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Fortified milk powders, infant formula and adult nutritionals. Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction

Published By	Publication Date	Number of Pages
BSI	2020	22

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Categories: 67.100.10 - Milk and processed milk products, BSI

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Description

This document specifies a method for the quantitative determination of biotin and/or biocytin in fortified milk powders, infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using liquid chromatography coupled with immunoaffinity column clean-up extraction.

Precision data from an interlaboratory study is given in Annex B. A comparison between data obtained with the method in this document and EN 15607 is given in Annex C.

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PDF Pages	PDF Title
2	National foreword
6	Foreword
7	1 Scope 2 Normative references 3 Terms and definitions 4 Principle
8	5 Reagents and materials 5.1 General 5.1.1 Laboratory reagent grade water. 5.1.2 Sodium dihydrogen phosphate dihydrate (CAS # 13472-35-0). 5.1.3 Disodium hydrogen phosphate dihydrate (CAS # 10028-24-7). 5.1.4 Sodium hydroxide (CAS #1310-73-2). 5.1.5 Methanol, HPLC grade (CAS # 67-56-1). 5.1.6 Acetonitrile, HPLC grade (CAS # 75-05-8). 5.1.7 Ortho-phosphoric acid, mass fraction w (H3PO4) = 85 % (CAS # 7664-38-2). 5.2 Reagent preparation
9	5.3 Standard preparation
10	5.4 Calculation of concentration 6 Apparatus 6.10 Horizontal shaker.
11	6.18 Vortex mixer. 7 Procedure 7.1 Sample preparation
12	7.2 Chromatography
13	7.3 Quality control 8 Calculations
14	9 Precision 9.1 General 9.2 Repeatability 9.3 Reproducibility 10 Uncertainty of measurement 11 Limit of quantitation
15	Annex A (informative) Example chromatograms
17	Annex B (informative) Precision data
19	Annex C (informative) Comparison between this document and EN 15607
21	Bibliography

Additional information

Status	Definitive
Pages	22
Publication Date	2020-04-21
ISBN	978 0 539 02692 4
Standard Number	BS ISO 23305:2020
Title	Fortified milk powders, infant formula and adult nutritionals. Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction
Identical National Standard Of	ISO 23305
Descriptors	Milk, Powder, Infant foods, Nutrition, LC (chromatography), Extraction methods of analysis, Test methods
Publisher	BSI
Committee	AW/34
ICS Codes	67.100.10 - Milk and processed milk products

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BS ISO 23305:2020

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