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BS ISO 19290:2016

BS ISO 19290:2016

$142.49

Cigarettes. Determination of tobacco specific nitrosamines in mainstream cigarette smoke. Method using LC-MS/MS

Published By Publication Date Number of Pages
BSI 2016 26
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PDF Catalog

PDF Pages PDF Title
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Apparatus
6 Reagents
11 7 Preparation
7.1 Preparation of glassware
7.2 Preparation of solutions
12 7.3 Preparation of standards
7.3.1 General
7.3.2 Preparation of internal standard solutions
7.3.3 Preparation of calibration standard solutions
14 8 Sampling
9 Tobacco product preparation
10 Sample generation — Smoking of cigarettes
10.1 General
10.2 Linear smoking
10.3 Rotary smoking
11 Sample analysis
11.1 Preparation of sample
11.1.1 General
11.1.2 Extraction for linear smoking (44 mm pad)
11.1.3 Extraction for rotary smoking (92 mm pad)
11.1.4 Final sample preparation
15 11.2 Reversed phase high performance liquid chromatography
11.2.1 General
11.2.2 HPLC set-up parameters (Example)
11.2.3 Mobile phase (Example)
11.2.4 Mobile phase: Gradient (Example)
11.2.5 MS/MS Set-up parameters (Example)
16 11.3 Calculation
11.3.1 Calibration curve
17 11.3.2 Determination of the TSNAs concentrations
11.3.3 Sample quantification
11.3.4 Determination of mainstream smoke TSNA deliveries
12 Repeatability and reproducibility
19 13 Test report
20 Annex A (informative) Example of typical chromatograms
22 Bibliography

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BS ISO 19290:2016
$142.49