BS ISO 19090:2018
$167.15
Tissue-engineered medical products. Bioactive ceramics. Method to measure cell migration in porous materials
| Published By | Publication Date | Number of Pages |
| BSI | 2018 | 42 |
The document specifies the test method to be followed for measuring and documenting the cell migration ability of porous bioactive ceramic materials.
This document is not applicable to porous materials that have low or no cell adhesion properties, for instance synthetic polymers and metals. These types of materials will require longer times to allow effective transfer and migration of cells from the cultured substrate to the test specimen. To minimize influences of cell passages, cell kinds, differences in cell culture consumables including culture medium and fetal bovine serum etc., the method uses a porous bioactive ceramics, which is clinically and widely used in each country, as a reference material for calculation of relative migration distance.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Principle |
| 11 | 5 Test specimen 5.1 Shape and dimensions 5.2 Number of test specimens 5.3 Reference material 6 Procedures 6.1 Measurement of thickness and diameter of specimen 6.2 Sterilization of test specimens 6.3 Deaeration of test specimens 6.4 Cell culture |
| 12 | 6.5 Placing specimens on the cell layer 6.6 Treatments after cell culture |
| 13 | 6.7 Positive and negative controls 6.8 Observations of cells that migrated into a specimen |
| 16 | 6.9 Measurement of migration distance of cells in a specimen 6.9.1 General 6.9.2 Maximum migration distance of cell |
| 19 | 6.10 Cells remaining on the well or dish after harvesting specimen 7 Calculation 7.1 Mean and standard deviation |
| 20 | 7.2 Relative mean and standard deviation by normalization 8 Test report |
| 22 | Annex A (informative) Results of tests for determination of test conditions |
| 34 | Annex B (informative) Results of international round robin test |
| 41 | Bibliography |
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ISO 19090:2018
Tissue-engineered medical products — Bioactive ceramics — Method to measure cell migration in porous materials…
