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BS EN ISO 24443:2012

$167.15

Determination of sunscreen UVA photoprotection in vitro

Published By Publication Date Number of Pages
BSI 2012 36
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This International Standard specifies an in vitro procedure to characterize the UVA protection of sunscreen products. Specifications are given to enable determination of the spectral absorbance characteristics of UVA protection in a reproducible manner.

In order to determine relevant UVA protection parameters, the method has been created to provide a UV spectral absorbance curve from which a number of calculations and evaluations can be undertaken. Results from this measurement procedure can be used for other computations, as required by local regulatory authorities. These include calculation of the Ultraviolet-A protection factor (UVAPF) [correlating with in vivo UVAPF from the persistent pigment darkening (PPD) testing procedure], critical wavelength and UVA absorbance proportionality. These computations are optional and relate to local sunscreen product labelling requirements. This method relies on the use of in vivo SPF results for scaling the UV absorbance curve.

This International Standard is not applicable to powder products such as pressed powder and loose powder products.

PDF Catalog

PDF Pages PDF Title
6 Foreword
7 1 Scope
2 Terms and definitions
8 3 Principle
4 Apparatus
4.1 UV spectrophotometer specifications
9 4.2 Calibration of the UV spectrophotometer
4.3 Calibration of the UV exposure source
4.4 Monitoring of the UV exposure source
10 4.5 Calibration of the UVA radiometer used to monitor the test sample irradiation
4.6 Substrate/plate
5 Test method
5.1 Outline of the test procedure
5.2 Equipment calibration and validation of test plates
11 5.3 Absorption measurements through the plate
5.4 Sample application
5.5 Absorbance measurements of the product-treated plate
5.6 Number of determinations
5.7 Determination of initial calculated SPF (SPFin vitro), “C” value, initial UVAPF(UVAPF0), and UV exposure dose
13 5.8 UV exposure
5.9 Measurement of final adjusted absorbance spectrum
14 5.10 Calculation of UVAPF of plates after UV exposure of the sample
6 Procedure using the spreadsheet in this International Standard
15 7 Reference sunscreen formula S2
8 Test report
16 Annex A
(normative)

Calibration of UV spectrophotometer and plate transmission test

20 Annex B
(normative)

Radiometer calibration to spectroradiometric irradiance procedure

22 Annex C
(normative)

Computation values: PPD and erythema action spectra and UVA
and UV-SSR spectral irradiances

25 Annex D
(normative)

PMMA test plate surface specifications

27 Annex E
(normative)

UVA reference sunscreen S2

31 Annex F
(informative)

Statistical calculations

33 Bibliography
Bibliography
BS EN ISO 24443:2012
$167.15