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BS EN ISO 22118:2011

$102.76

Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens. Performance characteristics

Published By Publication Date Number of Pages
BSI 2011 20
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This International Standard specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA or RNA) by molecular methods. This International Standard applies to the detection of food-borne pathogens in foodstuffs and isolates obtained from them using molecular detection methods based on the polymerase chain reaction (PCR).

This International Standard is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.

NOTE Because of the rapid progress in this field, the examples given are those most frequently in use at the time of development of this International Standard.

PDF Catalog

PDF Pages PDF Title
7 Scope
Normative references
Terms and definitions
9 Performance characteristics of qualitative and quantitative
General
Scope of the method
Scientific basis
Selectivity
Inclusivity test
General
Minimum requirements for specificity
Exclusivity test
General
10 Test systems for detecting bacteria
Test systems for detecting fungi
Test systems for detecting viruses
Sensitivity
General
Minimum requirements for sensitivity for qualitative tests
11 Minimum requirements for sensitivity for quantitative tests
Robustness
General
Robustness determination
Analytical controls
Trueness and precision
Instruments
Performance characteristics for validation
General
12 Determination of the limit of detection, limit of quantifica
Qualitative methods
General
False-positive rate
13 False-negative rate
Quantitative methods
Validation report
General
14 Qualitative methods
Quantitative methods
BS EN ISO 22118:2011
$102.76