BS EN ISO 14501:2021

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Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography

Published By	Publication Date	Number of Pages
BSI	2021	20

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Categories: 67.100.10 - Milk and processed milk products, BSI

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Description

This document specifies a method for the determination of aflatoxin M₁ content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder, i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 ?g/kg for milk powder and 0,005 ?g/kg for liquid milk. The limit of quantification (LOQ) is 0,1 ?g/kg for milk powder and 0,01 ?g/kg for liquid milk.

The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.

PDF Catalog

PDF Pages	PDF Title
2	National foreword
4	European foreword
6	Forewords
9	1 Scope 2 Normative references 3 Terms and definitions
10	4 Principle 5 Reagents 5.1 Immunoaffinity column.
11	5.3 Nitrogen gas. 5.4 Aflatoxin M1 standard solutions.
12	6 Apparatus
13	7 Sampling 8 Procedure 8.1 General 8.2 Preparation of test samples 8.2.1 Milk 8.2.2 Milk powder 8.3 Immunoaffinity column preparation
14	8.4 Test sample purification 8.5 High performance liquid chromatography (HPLC) 8.5.1 Pump setting 8.5.2 Chromatographic performance 8.5.3 Calibration curve of aflatoxin M1
15	8.5.4 Analysis of the purified extracts and injection scheme 9 Calculation and expression of results 9.1 Skimmed milk 9.1.1 Calculation 9.1.2 Expression of results 9.2 Skimmed milk powder 9.2.1 Calculation
16	9.2.2 Expression of results 10 Precision 10.1 Interlaboratory test 10.2 Repeatability 10.3 Reproducibility 11 Test report
17	Annex A (informative) Results of an interlaboratory trial
18	Bibliography

Additional information

Status	Definitive
Pages	20
Publication Date	2021-06-02
ISBN	978 0 539 06823 8
Standard Number	BS EN ISO 14501:2021
Title	Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography
Identical National Standard Of	EN ISO 14501, ISO 14501, EN ISO 14501:2021
Replaces	BS EN ISO 14501:2007
Descriptors	Immunoassay, Specimen preparation, Milk, Dried milk, Chemical analysis and testing, Food products, Determination of content, Test equipment, Test specimens, Aflatoxin, Affinity chromatography, Food testing, Liquid chromatography, Dairy products
Publisher	BSI
Committee	AW/5
ICS Codes	67.100.10 - Milk and processed milk products

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BS EN ISO 14501:2021

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