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ASTM-E2414:2005 Edition

ASTM-E2414:2005 Edition

$40.63

E2414-05 Standard Test Method for Quantitative Sporicidal Three-Step Method (TSM) to Determine Sporicidal Efficacy of Liquids, Liquid Sprays, and Vapor or Gases on Contaminated Carrier Surfaces

Published By Publication Date Number of Pages
ASTM 2005 9
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1.1 This test method determines the efficacy of sporicidal agents on microorganisms dried on the surface of solid carriers.

1.2 This test method can be used to evaluate sporicidal products (or decontaminant, disinfectant, and so forth), suspected, claimed, or assumed to have sporicidal activity. This test method allows:

1.2.1 Establishing the sporicidal efficacy of different disinfectants;

1.2.2 Identifying the effect, if any, of the surface materials on sporicidal efficacy; and

1.2.3 Comparing the relative resistance to disinfection of different microbial species or strains.

1.3 The values stated in SI units are to be regarded as the standard.

1.4 Strict adherence to the protocol is necessary for the validity of the test results.

1.5 Follow all the safety guidance of the institution in which the testing is being conducted. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages PDF Title
1 Scope
Referenced Documents
Summary of Test Method
Significance and Use
2 Apparatus
Reagents and Materials
Hazards
3 Sample Handling and Storage
Calibration and Standardization
Procedure
4 FIG. 1
FIG. 2
6 Calculation
Precision and Bias
Keywords
7 X1. PREPARATION OF BACILLUS SPORES IN LIQUID MEDIA
X1.1 Equipment
X1.2 Reagents
X1.3 Sporulation Media (Medium S)
X1.4 Spore-Growing Procedure
X1.5 Harvest and Rinses
8 X2. QUALITY CONTROL AND TITRATION OF SPORE PREPARATIONS
X2.1
X2.2
X2.3
X2.4
X2.5
X2.6
X3. HYDROCHLORIC TREATMENT
X3.1
X3.2
X3.3
X3.4
X3.5
X3.6
X3.7
X3.8
X4. NEUTRALIZER TESTING PROTOCOL
X4.1 Materials
X4.2 Preparation of Materials
X4.3 Method
9 REFERENCES

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