{"id":375949,"date":"2024-10-20T02:46:00","date_gmt":"2024-10-20T02:46:00","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-ts-17329-12021\/"},"modified":"2024-10-26T04:51:37","modified_gmt":"2024-10-26T04:51:37","slug":"bsi-pd-cen-ts-17329-12021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-ts-17329-12021\/","title":{"rendered":"BSI PD CEN\/TS 17329-1:2021"},"content":{"rendered":"

This document describes the performance characteristics and minimum performance criteria for conducting a single-laboratory validation study for qualitative (binary) real-time polymerase chain reaction (PCR) methods applied for the detection of specific DNA sequences present in foods.<\/p>\n

The protocol was developed for qualitative real-time PCR methods for the detection of DNA sequences derived from genetically modified foodstuffs. It is applicable also for single-laboratory validation of qualitative PCR methods used for analysis of other food materials, e.g. for species detection and identification.<\/p>\n

The document does not cover the evaluation of the applicability and the practicability with respect to the specific scope of the PCR method.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
5<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\nIntroduction <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
8<\/td>\n4 Principle
5 Single-laboratory validation of the performance characteristics
5.1 General
5.2 Limit of detection (LOD95 %) <\/td>\n<\/tr>\n
9<\/td>\n5.3 Evaluation of data for the limit of detection (LOD95 %)
5.4 PCR efficiency and variability of the measured copy number around the LOD95 % <\/td>\n<\/tr>\n
10<\/td>\n5.5 Specificity
5.5.1 General
5.5.2 Theoretical test for specificity
5.5.3 Practical test for specificity
5.5.4 Robustness <\/td>\n<\/tr>\n
11<\/td>\n6 Validation report <\/td>\n<\/tr>\n
12<\/td>\nAnnex A (informative) Estimation of the number of copies of the target sequence <\/td>\n<\/tr>\n
14<\/td>\nAnnex B (informative) Determination of limit of detection, precision and PCR efficiency <\/td>\n<\/tr>\n
17<\/td>\nAnnex C (informative) Statistical Model <\/td>\n<\/tr>\n
22<\/td>\nAnnex D (informative) Robustness testing <\/td>\n<\/tr>\n
24<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. General guidelines for the validation of qualitative real-time PCR methods – Single-laboratory validation<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2021<\/td>\n26<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":375958,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-375949","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/375949","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/375958"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=375949"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=375949"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=375949"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}