{"id":341507,"date":"2024-10-19T23:56:47","date_gmt":"2024-10-19T23:56:47","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-15216-22019\/"},"modified":"2024-10-25T23:09:16","modified_gmt":"2024-10-25T23:09:16","slug":"bs-en-iso-15216-22019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-15216-22019\/","title":{"rendered":"BS EN ISO 15216-2:2019"},"content":{"rendered":"

This document specifies a method for detection of hepatitis A virus (HAV) and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs [(soft fruit, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish (BMS)] or surfaces using real-time RT-PCR.<\/p>\n

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, surfaces or other matrices.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
9<\/td>\nForeword <\/td>\n<\/tr>\n
10<\/td>\nIntroduction <\/td>\n<\/tr>\n
11<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
13<\/td>\n4 Principle
4.1 Virus extraction
4.2 RNA extraction
4.3 Real-time RT-PCR <\/td>\n<\/tr>\n
14<\/td>\n4.4 Control materials
4.4.1 Process control virus
4.4.2 EC RNA control
4.5 Test results
5 Reagents
5.1 General
5.2 Reagents used as supplied <\/td>\n<\/tr>\n
16<\/td>\n5.3 Reagents requiring preparation <\/td>\n<\/tr>\n
17<\/td>\n6 Equipment and consumables <\/td>\n<\/tr>\n
18<\/td>\n7 Sampling
8 Procedure
8.1 General laboratory requirements
8.2 Virus extraction
8.2.1 General <\/td>\n<\/tr>\n
19<\/td>\n8.2.2 Process control virus material
8.2.3 Negative process control
8.2.4 Surfaces
8.2.5 Soft fruit and leaf, stem and bulb vegetables <\/td>\n<\/tr>\n
20<\/td>\n8.2.6 Bottled water
8.2.7 Bivalve molluscan shellfish (BMS) <\/td>\n<\/tr>\n
21<\/td>\n8.3 RNA extraction
8.4 Real-time RT-PCR
8.4.1 General requirements <\/td>\n<\/tr>\n
22<\/td>\n8.4.2 Real-time RT-PCR analysis <\/td>\n<\/tr>\n
24<\/td>\n9 Interpretation of results
9.1 General
9.2 Construction of process control virus RNA standard curve
9.3 Control for RT-PCR inhibition <\/td>\n<\/tr>\n
25<\/td>\n9.4 Calculation of extraction efficiency
10 Expression of results <\/td>\n<\/tr>\n
26<\/td>\n11 Performance characteristics of the method
11.1 Validation study
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
12 Test report <\/td>\n<\/tr>\n
27<\/td>\nAnnex A (normative) Diagram of procedure <\/td>\n<\/tr>\n
28<\/td>\nAnnex B (normative) Composition and preparation of reagents and buffers <\/td>\n<\/tr>\n
31<\/td>\nAnnex C (informative) Real-time RT-PCR mastermixes and cycling parameters <\/td>\n<\/tr>\n
32<\/td>\nAnnex D (informative) Real-time RT-PCR primers and hydrolysis probes for the detection of HAV, norovirus GI and GII and mengo virus (process control) <\/td>\n<\/tr>\n
35<\/td>\nAnnex E (informative) Growth of mengo virus strain MC0 for use as a process control <\/td>\n<\/tr>\n
36<\/td>\nAnnex F (informative) RNA extraction using the BioMerieux NucliSens\u00ae system <\/td>\n<\/tr>\n
38<\/td>\nAnnex G (informative) Generation of external control RNA (EC RNA) stocks <\/td>\n<\/tr>\n
41<\/td>\nAnnex H (informative) Typical optical plate layout <\/td>\n<\/tr>\n
42<\/td>\nAnnex I (informative) Method validation studies and performance characteristics <\/td>\n<\/tr>\n
50<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of the food chain. Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Method for detection<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2019<\/td>\n52<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":341516,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-341507","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/341507","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/341516"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=341507"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=341507"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=341507"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}