DIN EN ISO 15216-1:2021 Edition
$41.60
Mikrobiologie der Lebensmittelkette – Horizontales Verfahren zur Bestimmung von Hepatitis-A-Virus und Norovirus mittels Real-time-RT-PCR – Teil 1: Verfahren zur Quantifizierung
Published By | Publication Date | Number of Pages |
DIN | 2021-10 | 61 |
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In diesem Dokument wird ein Verfahren zur quantitativen Bestimmung des Gehalts an RNA von HAV und Noroviren der Genogruppen I (GI) und II (GII) in Untersuchungsproben von Lebensmitteln oder Lebensmitteloberflächen beschrieben. Nach der Freisetzung der Viren aus der Untersuchungsprobe wird die virale RNA durch Aufschluss des Viruskapsids mit Guanidinthiocyanat und Adsorption an Silica(-Partikeln) extrahiert. Die Zielsequenzen innerhalb der viralen RNA werden amplifiziert und mittels Real-time RT PCR nachgewiesen. Dieses Verfahren ist auch relevant für den Nachweis von Zielviren auf Virusträgern, oder von anderen Humanviren in Lebensmitteln, auf Lebensmitteloberflächen oder auf Virsuträgern nach entsprechender Validierung und unter Verwendung von ziel-spezifischen Primer- und Sondensätze.*Inhaltsverzeichnis
Published Code | DIN |
---|---|
Published By | Deutsches Institut für Normung |
Publication Date | 2021-10 |
Pages Count | 61 |
Language | Germany |
Descriptors | Analyseverfahren, Ansteckung, Bakterienviren, Begriffe, chemische Reaktion, Definition, Echtzeit, Futter, Futtermittel, Hepatitis, Kontamination, Laborprüfung, Laboruntersuchung, Lebensmittel, Lebensmittelprüfung, Lebensmitteluntersuchung, mikrobielle Lebensmittel, Mikrobiologie, mikrobiologische Analyse, Mikroorganismen, Nachweis, Nahrungsmittel, Norovirus, PCR, Polymerase, Probenahme, Prüfung, Prüfverfahren, quantitative Analyse, Tierfutter, Untersuchung, Virus |
File Size | 2.4 MB |
ICS Codes | 07.100.30 - Food microbiology |
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